The regenerated p lantlets of Acacia auriculiform is were obtained by the method of re2differentiation of thecallus from the hypocotyls exp lants. The effects of p lant regulator compositions on the induction of callus and thedifferentiation of adventitious buds in vitro culture were studied. The op timum medium for callus induction wasMSmedium containing 1. 0 or 1. 5 mg ·L-1 2, 42D and 0. 5 mg ·L-1 KT and 100% the callus induction frequencywasobtained. The op timum medium for re2differentiation of calluswasMSmedium containing 1. 5 mg ·L-1 62BA and0. 2 mg ·L-1 NAA and 84. 7% of adventitious shoot regeneration frequency with 5. 83 shoots per exp lants wasobtained. In addition, the op timum medium for the adventitious buds induced from the hypocotyls exp lants withouttransferring the calluswasMSmedium containing 2. 0 mg ·L-1 62BA, 0. 1 mg ·L-1 NAA and 0. 2 mg ·L-1 KT.Excised shootswere effectively elongated inMS medium without appending any hormones. Elongated shoots of 3. 0cm rooted when theywere transferred toMSmedium containing 0. 1 mg ·L-1 IAA and 0. 2 mg ·L-1 NAA after 30days and developed into healthy p lantlets, which resulted in a rooting rate of 85 %. The results of this study willfacilitate the app lication of genetic transformation methods in A. auricu liform is.

香石竹ACC氧化酶基因从核NDA中克隆之后,将其首先构建成正义及反义的单拷贝植物表达载体,在此基础上又同向插入一个ACO基因片段,从而获得ACO的正义重复基因和反义重复基因植物表达载体,所得载体已通过酶切分析和PCR鉴定。将该表达载体导入根癌农杆菌LBA4404菌株,并转化香石竹品种Master、爱卡迪幼叶,经PCR检测和Southern杂交鉴定,获得了8株转化植珠。